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The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II.

ABSTRACT: CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

SUBMITTER: Elmlund H 

PROVIDER: S-EPMC1635081 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature

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The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II.

Elmlund Hans H   Baraznenok Vera V   Lindahl Martin M   Samuelsen Camilla O CO   Koeck Philip J B PJ   Holmberg Steen S   Hebert Hans H   Gustafsson Claes M CM  

Proceedings of the National Academy of Sciences of the United States of America 20061016 43

CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Media  ...[more]

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