Pathway-specific differences between tumor cell lines and normal and tumor tissue cells.

ABSTRACT:

Background

Cell lines are used in experimental investigation of cancer but their capacity to represent tumor cells has yet to be quantified. The aim of the study was to identify significant alterations in pathway usage in cell lines in comparison with normal and tumor tissue.

Methods

This study utilized a pathway-specific enrichment analysis of publicly accessible microarray data and quantified the gene expression differences between cell lines, tumor, and normal tissue cells for six different tissue types. KEGG pathways that are significantly different between cell lines and tumors, cell lines and normal tissues and tumor and normal tissue were identified through enrichment tests on gene lists obtained using Significance Analysis of Microarrays (SAM).

Results

Cellula

SUBMITTER: Ertel A 

PROVIDER: S-EPMC1635729 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature