Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy.

ABSTRACT: Combinations of molecular tags visible in light and electron microscopes become particularly advantageous in the analysis of dynamic cellular components like the Golgi apparatus. This organelle disassembles at the onset of mitosis and, after a sequence of poorly understood events, reassembles after cytokinesis. The precise location of Golgi membranes and resident proteins during mitosis remains unclear, partly due to limitations of molecular markers and the resolution of light microscopy. We generated a fusion consisting of the first 117 residues of alpha-mannosidase II tagged with a fluorescent protein and a tetracysteine motif. The mannosidase component guarantees docking into the Golgi membrane, with the tags exposed in the lumen. The fluorescent protein is optically visible without fur

SUBMITTER: Gaietta GM 

PROVIDER: S-EPMC1635977 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature