A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation.

ABSTRACT:

Background

The floral dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium is the method of choice for Arabidopsis transformation. The presence of a marker, usually antibiotic- or herbicide-resistance, allows identification of transformed seedlings from untransformed seedlings. Seedling selection is a lengthy process which does not always lead to easily identifiable transformants. Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants.

Results

A method for identifying transformed seedlings in as little as 3.25 d has been developed. Arabidopsis T1 seeds obtained after floral dip transformation are plat

SUBMITTER: Harrison SJ 

PROVIDER: S-EPMC1636043 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature