Converting a breast cancer microarray signature into a high-throughput diagnostic test.

ABSTRACT:

Background

A 70-gene tumor expression profile was established as a powerful predictor of disease outcome in young breast cancer patients. This profile, however, was generated on microarrays containing 25,000 60-mer oligonucleotides that are not designed for processing of many samples on a routine basis.

Results

To facilitate its use in a diagnostic setting, the 70-gene prognosis profile was translated into a customized microarray (MammaPrint) containing a reduced set of 1,900 probes suitable for high throughput processing. RNA of 162 patient samples from two previous studies was subjected to hybridization to this custom array to validate the prognostic value. Classification results obtained from the original analysis were then compared to those generated using the algorithms

SUBMITTER: Glas AM 

PROVIDER: S-EPMC1636049 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature