Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.

ABSTRACT: In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.

SUBMITTER: Klijn A 

PROVIDER: S-EPMC1636199 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature