Screening for plant transporter function by expressing a normalized Arabidopsis full-length cDNA library in Xenopus oocytes.

ABSTRACT:

Background

We have developed a functional genomics approach based on expression cloning in Xenopus oocytes to identify plant transporter function. We utilized the full-length cDNA databases to generate a normalized library consisting of 239 full-length Arabidopsis thaliana transporter cDNAs. The genes were arranged into a 96-well format and optimized for expression in Xenopus oocytes by cloning each coding sequence into a Xenopus expression vector.

Results

Injection of 96 in vitro transcribed cRNAs from the library in pools of columns and rows into oocytes and subsequent screening for glucose uptake activity identified three glucose transporters. One of these, AtSTP13, had not previously been experimentally characterized.

Conclusion

Expression of the library in Xenopu

SUBMITTER: Nour-Eldin HH 

PROVIDER: S-EPMC1637106 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature