Complement lysis activity in autologous plasma is associated with lower viral loads during the acute phase of HIV-1 infection.

ABSTRACT:

Background

To explore the possibility that antibody-mediated complement lysis contributes to viremia control in HIV-1 infection, we measured the activity of patient plasma in mediating complement lysis of autologous primary virus.

Methods and findings

Sera from two groups of patients-25 with acute HIV-1 infection and 31 with chronic infection-were used in this study. We developed a novel real-time PCR-based assay strategy that allows reliable and sensitive quantification of virus lysis by complement. Plasma derived at the time of virus isolation induced complement lysis of the autologous virus isolate in the majority of patients. Overall lysis activity against the autologous virus and the heterologous primary virus strain JR-FL was higher at chronic disease stages than durin

SUBMITTER: Huber M 

PROVIDER: S-EPMC1637124 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature