A high-throughput method for cloning and sequencing human immunodeficiency virus type 1 integration sites.

ABSTRACT: Integration of retroviral DNA is nonspecific and can occur at many sites throughout chromosomes. However, the process is not uniformly distributed, and both hot and cold spots for integration exist. The mechanism that determines target site specificity is not well understood. Because of the nonspecific and widespread nature of integration, studies analyzing the mechanism and factors that control target site selection require the collection and analysis of a large library of human immunodeficiency virus type 1 (HIV-1) proviral clones. Such analyses are time-consuming and labor-intensive using conventional means. We have developed an efficient and high-throughput method of sequencing and mapping a large number of independent integration sites in the absence of any selection or bias. The new

SUBMITTER: Kim S 

PROVIDER: S-EPMC1642152 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature