Developing an energy landscape for the novel function of a (beta/alpha)8 barrel: ammonia conduction through HisF.

ABSTRACT: HisH-hisF is a multidomain globular protein complex; hisH is a class I glutamine amidotransferase that hydrolyzes glutamine to form ammonia, and hisF is a (beta/alpha)8 barrel cyclase that completes the ring formation of imidizole glycerol phosphate synthase. Together, hisH and hisF form a glutamine amidotransferase that carries out the fifth step of the histidine biosynthetic pathway. Recently, it has been suggested that the (beta/alpha)8 barrel participates in a novel function: to channel ammonia from the active site of hisH to the active site of hisF. The present study presents a series of molecular dynamic simulations that investigate the channeling function of hisF. This article reconstructs potentials of mean force for the conduction of ammonia through the channel, and the entrance o

SUBMITTER: Amaro R 

PROVIDER: S-EPMC164632 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature