PiggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila.

ABSTRACT: P element-mediated mutagenesis has been used to disrupt an estimated 25% of genes essential for Drosophila adult viability. Mutation of all genes in the fly genome, however, poses a problem, because P elements show significant hotspots of integration. In addition, advanced screening scenarios often require the use of P element-based tools like the generation of germ-line mosaics using FLP recombinase-mediated recombination or gene misexpression using the UAS/Gal4 system. These techniques are P element-based and can therefore not be combined with the use of P elements as mutagenic agents. To circumvent these limitations, we have developed an insertional mutagenesis system using non-P element transposons. An enhanced yellow fluorescent protein-marked piggyBac-based mutator element was mobili

SUBMITTER: Hacker U 

PROVIDER: S-EPMC164654 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature