Human gene targeting by adeno-associated virus vectors is enhanced by DNA double-strand breaks.

ABSTRACT: The use of adeno-associated virus (AAV) to package gene-targeting vectors as single-stranded linear molecules has led to significant improvements in mammalian gene-targeting frequencies. However, the molecular basis for the high targeting frequencies obtained is poorly understood, and there could be important mechanistic differences between AAV-mediated gene targeting and conventional gene targeting with transfected double-stranded DNA constructs. Conventional gene targeting is thought to occur by the double-strand break (DSB) model of homologous recombination, as this can explain the higher targeting frequencies observed when DSBs are present in the targeting construct or target locus. Here we compare AAV-mediated gene-targeting frequencies in the presence and absence of induced target si

SUBMITTER: Miller DG 

PROVIDER: S-EPMC164770 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature