Environmental isolates of Aeromonas spp. harboring the cagA-like gene of Helicobacter pylori.

ABSTRACT: We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.

SUBMITTER: Datta S 

PROVIDER: S-EPMC165152 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature