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Detection of Legionella pneumophila by real-time PCR for the mip gene.

ABSTRACT: A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.

SUBMITTER: Wilson DA 

PROVIDER: S-EPMC165371 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature

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Detection of Legionella pneumophila by real-time PCR for the mip gene.

Wilson Deborah A DA   Yen-Lieberman Belinda B   Reischl Udo U   Gordon Steve M SM   Procop Gary W GW  

Journal of clinical microbiology 20030701 7

A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila. ...[more]

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