Differential gene expression in the murine gastric fundus lacking interstitial cells of Cajal.

ABSTRACT:

Background

The muscle layers of murine gastric fundus have no interstitial cells of Cajal at the level of the myenteric plexus and only possess intramuscular interstitial cells and this tissue does not generate electric slow waves. The absence of intramuscular interstitial cells in W/WV mutants provides a unique opportunity to study the molecular changes that are associated with the loss of these intercalating cells.

Method

The gene expression profile of the gastric fundus of wild type and W/WV mice was assayed by murine microarray analysis displaying a total of 8734 elements. Queried genes from the microarray analysis were confirmed by semi-quantitative reverse transcription-polymerase chain reaction.

Results

Twenty-one genes were differentially expressed in wild typ

SUBMITTER: Daigo Y 

PROVIDER: S-EPMC165421 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature