Reproducibility of gene expression across generations of Affymetrix microarrays.

ABSTRACT:

Background

The development of large-scale gene expression profiling technologies is rapidly changing the norms of biological investigation. But the rapid pace of change itself presents challenges. Commercial microarrays are regularly modified to incorporate new genes and improved target sequences. Although the ability to compare datasets across generations is crucial for any long-term research project, to date no means to allow such comparisons have been developed. In this study the reproducibility of gene expression levels across two generations of Affymetrix GeneChips (HuGeneFL and HG-U95A) was measured.

Results

Correlation coefficients were computed for gene expression values across chip generations based on different measures of similarity. Comparing the absolute calls a

SUBMITTER: Nimgaonkar A 

PROVIDER: S-EPMC165600 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature