Confirmation of tick bite by detection of antibody to Ixodes calreticulin salivary protein.

ABSTRACT: Ticks introduce a variety of pharmacologically active molecules into their host during attachment and feeding in order to obtain a blood meal. People who are repeatedly exposed to ticks may develop an immune response to tick salivary proteins. Despite this response, people usually are unaware of having been bitten, especially if they are not repeatedly exposed to ticks. In order to develop a laboratory marker of tick exposure that would be useful in understanding the epidemiology of tick-borne infection and the immune response to tick bite, we developed an enzyme-linked immunosorbent assay (ELISA) to detect antibody to a recombinant form of calreticulin protein found in the salivary glands of Ixodes scapularis, a member of a complex of Ixodes ticks that serve as the vectors for Lyme diseas

SUBMITTER: Alarcon-Chaidez F 

PROVIDER: S-EPMC1656538 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature