Characterizing exons 11 and 1 promoters of the mu opioid receptor (Oprm) gene in transgenic mice.

ABSTRACT:

Background

The complexity of the mouse mu opioid receptor (Oprm) gene was demonstrated by the identification of multiple alternatively spliced variants and promoters. Our previous studies have identified a novel promoter, exon 11 (E11) promoter, in the mouse Oprm gene. The E11 promoter is located approximately 10 kb upstream of the exon 1 (E1) promoter. The E11 promoter controls the expression of nine splice variants in the mouse Oprm gene. Distinguished from the TATA-less E1 promoter, the E11 promoter resembles a typical TATA-containing eukaryote class II promoter. The aim of this study is to further characterize the E11 and E1 promoters in vivo using a transgenic mouse model.

Results

We constructed a approximately 20 kb transgenic construct in which a 3.7 kb E11 promoter r

SUBMITTER: Xu J 

PROVIDER: S-EPMC1657025 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature