Acetylation-dependent chromatin reorganization by BRDT, a testis-specific bromodomain-containing protein.

ABSTRACT: The association between histone acetylation and replacement observed during spermatogenesis prompted us to consider the testis as a source for potential factors capable of remodelling acetylated chromatin. A systematic search of data banks for open reading frames encoding testis-specific bromodomain-containing proteins focused our attention on BRDT, a testis-specific protein of unknown function containing two bromodomains. BRDT specifically binds hyperacetylated histone H4 tail depending on the integrity of both bromodomains. Moreover, in somatic cells, the ectopic expression of BRDT triggered a dramatic reorganization of the chromatin only after induction of histone hyperacetylation by trichostatin A (TSA). We then defined critical domains of BRDT involved in its activity. Both bromodomai

SUBMITTER: Pivot-Pajot C 

PROVIDER: S-EPMC165724 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature