Genotoxicants target distinct molecular networks in neonatal neurons.

ABSTRACT:

Background

Exposure of the brain to environmental agents during critical periods of neuronal development is considered a key factor underlying many neurologic disorders.

Objectives

In this study we examined the influence of genotoxicants on cerebellar function during early development by measuring global gene expression changes.

Methods

We measured global gene expression in immature cerebellar neurons (i.e., granule cells) after treatment with two distinct alkylating agents, methylazoxymethanol (MAM) and nitrogen mustard (HN2). Granule cell cultures were treated for 24 hr with MAM (10-1,000 microM) or HN2 (0.1-20 microM) and examined for cell viability, DNA damage, and markers of apoptosis.

Results

Neuronal viability was significantly reduced (p < 0.01) at conc

SUBMITTER: Kisby GE 

PROVIDER: S-EPMC1665395 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature