Reovirus-induced alteration in expression of apoptosis and DNA repair genes with potential roles in viral pathogenesis.

ABSTRACT: Reoviruses are a leading model for understanding cellular mechanisms of virus-induced apoptosis. Reoviruses induce apoptosis in multiple cell lines in vitro, and apoptosis plays a key role in virus-induced tissue injury of the heart and brain in vivo. The activation of transcription factors NF-kappaB and c-Jun are key events in reovirus-induced apoptosis, indicating that new gene expression is critical to this process. We used high-density oligonucleotide microarrays to analyze cellular transcriptional alterations in HEK293 cells after infection with reovirus strain T3A (i.e., apoptosis inducing) compared to infection with reovirus strain T1L (i.e., minimally apoptosis inducing) and uninfected cells. These strains also differ dramatically in their potential to induce apoptotic injury in he

SUBMITTER: DeBiasi RL 

PROVIDER: S-EPMC167209 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature