Detecting statistically significant common insertion sites in retroviral insertional mutagenesis screens.

ABSTRACT: Retroviral insertional mutagenesis screens, which identify genes involved in tumor development in mice, have yielded a substantial number of retroviral integration sites, and this number is expected to grow substantially due to the introduction of high-throughput screening techniques. The data of various retroviral insertional mutagenesis screens are compiled in the publicly available Retroviral Tagged Cancer Gene Database (RTCGD). Integrally analyzing these screens for the presence of common insertion sites (CISs, i.e., regions in the genome that have been hit by viral insertions in multiple independent tumors significantly more than expected by chance) requires an approach that corrects for the increased probability of finding false CISs as the amount of available data increases. Moreove

SUBMITTER: de Ridder J 

PROVIDER: S-EPMC1676030 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature