Streptomyces-derived quorum-sensing systems engineered for adjustable transgene expression in mammalian cells and mice.

ABSTRACT: Prokaryotic transcriptional regulatory elements have been adopted for controlled expression of cloned genes in mammalian cells and animals, the cornerstone for gene-function correlations, drug discovery, biopharmaceutical manufacturing as well as advanced gene therapy and tissue engineering. Many prokaryotes have evolved specific molecular communication systems known as quorum-sensing to coordinate population-wide responses to physiological and/or physicochemical signals. A generic bacterial quorum-sensing system is based on a diffusible signal molecule that prevents binding of a repressor to corresponding operator sites thus resulting in derepression of a target regulon. In Streptomyces, a family of butyrolactones and their corresponding receptor proteins, serve as quorum-sensing systems

SUBMITTER: Weber W 

PROVIDER: S-EPMC167645 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature