Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis.

ABSTRACT: Real-time PCR is being used increasingly as the method of choice for mRNA quantification, allowing rapid analysis of gene expression from low quantities of starting template. Despite a wide range of approaches, the same principles underlie all data analysis, with standard approaches broadly classified as either absolute or relative. In this study we use a variety of absolute and relative approaches of data analysis to investigate nocturnal c-fos expression in wild-type and retinally degenerate mice. In addition, we apply a simple algorithm to calculate the amplification efficiency of every sample from its amplification profile. We confirm that nocturnal c-fos expression in the rodent eye originates from the photoreceptor layer, with around a 5-fold reduction in nocturnal c-fos expression i

SUBMITTER: Peirson SN 

PROVIDER: S-EPMC167648 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature