DNA replication origin interference increases the spacing between initiation events in human cells.

ABSTRACT: Mammalian DNA replication origins localize to sites that range from base pairs to tens of kilobases. A regular distribution of initiations in individual cell cycles suggests that only a limited number of these numerous potential start sites are converted into activated origins. Origin interference can silence redundant origins; however, it is currently unknown whether interference participates in spacing functional human initiation events. By using a novel hybridization strategy, genomic Morse code, on single combed DNA molecules from primary keratinocytes, we report the initiation sites present on 1.5 Mb of human chromosome 14q11.2. We confirm that initiation zones are widespread in human cells, map to intergenic regions, and contain sequence motifs found at other mammalian initiation zon

SUBMITTER: Lebofsky R 

PROVIDER: S-EPMC1679695 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature