Monitoring dynamics of single-cell gene expression over multiple cell cycles.

ABSTRACT: Recent progress in reconstructing gene regulatory networks has established a framework for a quantitative description of the dynamics of many important cellular processes. Such a description will require novel experimental techniques that enable the generation of time-series data for the governing regulatory proteins in a large number of individual living cells. Here, we utilize microfabrication to construct a Tesla microchemostat that permits single-cell fluorescence imaging of gene expression over many cellular generations. The device is used to capture and constrain asymmetrically dividing or motile cells within a trapping region and to deliver nutrients and regulate the cellular population within this region. We illustrate the operation of the microchemostat with Saccharomyces cerevisi

SUBMITTER: Cookson S 

PROVIDER: S-EPMC1681470 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature