Core transcriptional regulatory circuitry in human hepatocytes.

ABSTRACT: We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.

SUBMITTER: Odom DT 

PROVIDER: S-EPMC1681491 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature