Cloning and expression of a novel human antibody-antigen pair associated with Felty's syndrome.

ABSTRACT: An increasing number of studies suggest the importance of antibodies in the pathogenesis of most systemic and organ-specific autoimmune diseases, although there is considerable controversy over the precise role of the autoantibodies involved. In humans, a major obstacle to progress is the identification and cloning of the relevant autoantibodies and autoantigens. Here, an approach based on the sequential use of antibody phage display and antigen expression libraries is developed and applied to a donor suffering from rheumatoid arthritis (RA), splenomegaly, and peripheral destruction of neutrophils leading to neutropenia (Felty's syndrome). An antibody phage display library was constructed from bone marrow from the donor and a high-affinity human mAb, ANA15, selected by panning against fres

SUBMITTER: Ditzel HJ 

PROVIDER: S-EPMC16851 | biostudies-literature | 2000 Aug

REPOSITORIES: biostudies-literature