High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium.

ABSTRACT:

Background

During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of beta-lactam antibiotics were systematically improved.

Results

For this purpose, the PGA leader peptide was replaced by the B. megaterium LipA counterpart. A production strain deficient in the extracellular protease NprM and in xylose utilization to prevent gene inducer deprivation was constructed and employed. A buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production was developed in microscale cultivations and scaled up to a 2 Liter bioreactor

SUBMITTER: Yang Y 

PROVIDER: S-EPMC1687198 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature