OspA heterogeneity of Borrelia valaisiana confirmed by phenotypic and genotypic analyses.

ABSTRACT:

Background

Although European Borrelia burgdorferi sensu lato isolates have been divided into five genospecies, specific tools for the serotype characterization of only three genospecies are available. Monoclonals antibodies (mAbs) H3TS, D6 and I17.3 identify B. burgdorferi sensu stricto (ss.), B. garinii and B. afzelii respectively, but no mAbs are available to identify B. valaisiana. In the same way, specific primers exist to amplify the OspA gene of B. burgdorferi ss., B. garinii and B. afzelii. The aim of the study was to develop species-specific mAb and PCR primers for the phenotypic and genetic identification of B. valaisiana.

Results

This study describes a mAb that targets OspA of B. valaisiana and primers targeting the OspA gene of this species. As the monoclonal anti

SUBMITTER: Ryffel K 

PROVIDER: S-EPMC169163 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature