Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation.

ABSTRACT: The filamentous soil bacterium Streptomyces coelicolor undergoes a complex cycle of morphological differentiation involving the formation of an aerial mycelium and the production of pigmented antibiotics. We have developed a procedure for generating insertional mutants of S. coelicolor based on in vitro transposition of a plasmid library of cloned S. coelicolor DNAs. The insertionally mutated library was introduced into S. coelicolor, and transposon insertions were recovered at widely scattered locations around the chromosome. Many of the insertions revealed previously uncharacterized genes, and several caused novel mutant phenotypes, such as altered pigment production, enhanced antibiotic sensitivity, delayed or impaired formation of aerial hyphae, and a block in spore formation. The spor

SUBMITTER: Gehring AM 

PROVIDER: S-EPMC16918 | biostudies-literature | 2000 Aug

REPOSITORIES: biostudies-literature