Reconstructing repressor protein levels from expression of gene targets in Escherichia coli.

ABSTRACT: The basic underlying problem in reverse engineering of gene regulatory networks from gene expression data is that the expression of a gene encoding the regulator provides only limited information about its protein activity. The proteins, which result from translation, are subject to stringent posttranscriptional control and modification. Often, it is only the modified version of the protein that is capable of activating or repressing its regulatory targets. At present there exists no reliable high-throughput technology to measure the protein activity levels in real-time, and therefore they are, so-to-say, lost in translation. However, these activity levels can be recovered by studying the gene expression of their targets. Here, we describe a computational approach to predict temporal regul

SUBMITTER: Khanin R 

PROVIDER: S-EPMC1693707 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature