The mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration.

ABSTRACT: The model bryophyte Physcomitrella patens exhibits high frequencies of gene targeting when transformed with DNA constructs containing sequences homologous with genomic loci. 'Targeted gene replacement' (TGR) resulting from homologous recombination (HR) between each end of a targeting construct and the targeted locus occurs when either single or multiple targeting vectors are delivered. In the latter instance simultaneous, multiple, independent integration of different transgenes occurs at the targeted loci. In both single gene and 'batch' transformations, DNA can also be found to undergo 'targeted insertion' (TI), integrating at one end of the targeted locus by HR with one flanking sequence of the vector accompanied by an apparent non-homologous end-joining (NHEJ) event at the other. Untar

SUBMITTER: Kamisugi Y 

PROVIDER: S-EPMC1693892 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature