Genomewide identification of pheromone-targeted transcription in fission yeast.

ABSTRACT:

Background

Fission yeast cells undergo sexual differentiation in response to nitrogen starvation. In this process haploid M and P cells first mate to form diploid zygotes, which then enter meiosis and sporulate. Prior to mating, M and P cells communicate with diffusible mating pheromones that activate a signal transduction pathway in the opposite cell type. The pheromone signalling orchestrates mating and is also required for entry into meiosis.

Results

Here we use DNA microarrays to identify genes that are induced by M-factor in P cells and by P-factor in M-cells. The use of a cyr1 genetic background allowed us to study pheromone signalling independently of nitrogen starvation. We identified a total of 163 genes that were consistently induced more than two-fold by pheromone

SUBMITTER: Xue-Franzen Y 

PROVIDER: S-EPMC1693924 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature