Validation of microsatellite markers for use in genotyping polyclonal Plasmodium falciparum infections.

ABSTRACT: Genotyping methods for Plasmodium falciparum drug efficacy trials have not been standardized and may fail to accurately distinguish recrudescence from new infection, especially in high transmission areas where polyclonal infections are common. We developed a simple method for genotyping using previously identified microsatellites and capillary electrophoresis, validated this method using mixtures of laboratory clones, and applied the method to field samples. Two microsatellite markers produced accurate results for single-clone but not polyclonal samples. Four other microsatellite markers were as sensitive as, and more specific than, commonly used genotyping techniques based on merozoite surface proteins 1 and 2. When applied to samples from 15 patients in Burkina Faso with recurrent parasi

SUBMITTER: Greenhouse B 

PROVIDER: S-EPMC1697796 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature