Protein phase diagrams II: nonideal behavior of biochemical reactions in the presence of osmolytes.

ABSTRACT: In the age of biochemical systems biology, proteomics, and high throughput methods, the thermodynamic quantification of cytoplasmatic reaction networks comes into reach of the current generation of scientists. What is needed to efficiently extract the relevant information from the raw data is a robust tool for evaluating the number and stoichiometry of all observed reactions while providing a good estimate of the thermodynamic parameters that determine the molecular behavior. The recently developed phase-diagram method, strictly speaking a graphical representation of linkage or Maxwell Relations, offers such capabilities. Here, we extend the phase diagram method to nonideal conditions. For the sake of simplicity, we choose as an example a reaction system involving the protein RNase A, its

SUBMITTER: Ferreon AC 

PROVIDER: S-EPMC1697851 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature