Integrated siRNA design based on surveying of features associated with high RNAi effectiveness.

ABSTRACT:

Background

Short interfering RNAs have allowed the development of clean and easily regulated methods for disruption of gene expression. However, while these methods continue to grow in popularity, designing effective siRNA experiments can be challenging. The various existing siRNA design guidelines suffer from two problems: they differ considerably from each other, and they produce high levels of false-positive predictions when tested on data of independent origins.

Results

Using a distinctly large set of siRNA efficacy data assembled from a vast diversity of origins (the siRecords data, containing records of 3,277 siRNA experiments targeting 1,518 genes, derived from 1,417 independent studies), we conducted extensive analyses of all known features that have been implicated

SUBMITTER: Gong W 

PROVIDER: S-EPMC1698580 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature