Functional analysis of genes differentially expressed in the Drosophila wing disc: role of transcripts enriched in the wing region.

ABSTRACT: Differential gene expression is the major mechanism underlying the development of specific body regions. Here we assessed the role of genes differentially expressed in the Drosophila wing imaginal disc, which gives rise to two distinct adult structures: the body wall and the wing. Reverse genetics was used to test the function of uncharacterized genes first identified in a microarray screen as having high levels of expression in the presumptive wing. Such genes could participate in elaborating the specific morphological characteristics of the wing. The activity of the genes was modulated using misexpression and RNAi-mediated silencing. Misexpression of eight of nine genes tested caused phenotypes. Of 12 genes tested, 10 showed effective silencing with RNAi transgenes, but only 3 of these h

SUBMITTER: Jacobsen TL 

PROVIDER: S-EPMC1698657 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature