Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts.

ABSTRACT:

Background

The replication of mammalian genomic DNA during the S phase is a highly coordinated process that occurs in a programmed manner. Recent studies have begun to elucidate the pattern of replication timing on a genomic scale. Using a combination of experimental and computational techniques, we identified a genome-wide set of the earliest replicating sequences. This was accomplished by first creating a cosmid library containing DNA enriched in sequences that replicate early in the S phase of normal human fibroblasts. Clone ends were then sequenced and aligned to the human genome.

Results

By clustering adjacent or overlapping early replicating clones, we identified 1759 "islands" averaging 100 kb in length, allowing us to perform the most detailed analysis to date of DNA

SUBMITTER: Cohen SM 

PROVIDER: S-EPMC1702361 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature