Identification of metastasis-associated proteins in a human tumor metastasis model using the mass-mapping technique.

ABSTRACT: For most cancer cell types, the acquisition of metastatic ability leads to clinically incurable disease. The identification of molecules whose expression is specifically correlated with the metastatic spread of cancer would facilitate the design of therapeutic interventions to inhibit this lethal process. In order to facilitate metastasis gene discovery we have previously characterized a pair of monoclonal cell lines from the human breast carcinoma cell line MDA-MB-435 that have different metastatic phenotypes in immune-compromised mice. In this study, serum-free conditioned media was collected from the cultured monoclonal cell lines and a mass mapping technique was applied in order to profile a component of each cell line proteome. We utilized chromatofocusing in the first dimension to ob

SUBMITTER: Kreunin P 

PROVIDER: S-EPMC1702405 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature