Whole blood gene expression in infants with respiratory syncytial virus bronchiolitis.

ABSTRACT:

Background

Respiratory syncytial virus (RSV) is a major cause of viral bronchiolitis in infants worldwide, and environmental, viral and host factors are all of importance for disease susceptibility and severity. To study the systemic host response to this disease we used the microarray technology to measure mRNA gene expression levels in whole blood of five male infants hospitalised with acute RSV, subtype B, bronchiolitis versus five one year old male controls exposed to RSV during infancy without bronchiolitis. The gene expression levels were further evaluated in a new experiment using quantitative real-time polymerase chain reaction (QRT-PCR) both in the five infants selected for microarray and in 13 other infants hospitalised with the same disease.

Results

Among the 30 g

SUBMITTER: Fjaerli HO 

PROVIDER: S-EPMC1713240 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature