Analysis of iceA genotypes in South African Helicobacter pylori strains and relationship to clinically significant disease.

ABSTRACT:

Background

South African Helicobacter pylori isolates are characterised by the universal presence of cagA but have differences in vacuolating cytotoxin gene (vacA) alleles which correlate with clinically significant disease. However, the candidate virulence marker gene iceA has not been investigated.

Aim

To characterise the genetic organisation and heterogeneity of iceA genotypes in different South African clinical isolates.

Patients and methods

We studied H pylori strains isolated from 86 dyspeptic patients (30 with peptic ulcer disease (PUD), 19 with distal gastric adenocarcinoma (GC), and 37 with non-erosive gastritis) for the presence of iceA1 or iceA2 genes, and for differences in the genetic organisation of iceA2 by polymerase chain reaction, Southern hybridisat

SUBMITTER: Kidd M 

PROVIDER: S-EPMC1728508 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature