Immunohistochemistry for MSH2 and MHL1: a method for identifying mismatch repair deficient colorectal cancer.

ABSTRACT: Colorectal cancers with DNA mismatch repair (MMR) gene mutations characteristically display a high rate of replication errors in simple repetitive sequences detectable as microsatellite instability (MSI). Most are the result of somatic MMR dysfunction; however, a subset are caused by germline mutations. The availability of commercial antibodies for MSH2 and MLH1 [corrected] offers an alternative strategy to molecular methods for identifying MMR deficient cancers. To evaluate immunohistochemistry, MLH1 and MSH2 expression was studied using monoclonal antibodies in formalin fixed, paraffin wax embedded cancers. The immunohistochemical staining patterns of 23 cancers displaying MSI, including four cases with germline mutations, were compared with 23 microsatellite stable (MSS) cancers. All MS

SUBMITTER: Stone JG 

PROVIDER: S-EPMC1731447 | biostudies-literature | 2001 Jun

REPOSITORIES: biostudies-literature