Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos.

ABSTRACT: Global activation of the embryonic genome is the most critical event in early mammalian development. After fertilization, a rich supply of maternal proteins and RNAs support development whereas a number of zygotic and embryonic genes are expressed in a stage-specific manner leading to embryonic genome activation (EGA). However, the identities of embryonic genes expressed and the mechanism(s) of EGA are poorly defined in the bovine. Using the Affymetrix bovine-specific DNA microarray as the biggest available array at present, we analyzed gene expression at two key stages of bovine development, matured oocytes (MII) and 8-cell-stage embryos, constituting the ultimate reservoir for life and a stage during which EGA takes place, respectively. Key genes in regulation of transcription, chromatin

SUBMITTER: Misirlioglu M 

PROVIDER: S-EPMC1748150 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature