Metabolic activation stimulates acid secretion and expression of matrix degrading proteases in human osteoblasts.

ABSTRACT:

Background

Both cellular and matrix components of healthy bone are permanently renewed in a balanced homoeostasis. Osteoclastic bone resorption involves the expression of vacuolar-type ATPase proton pumps (vATPase) on the outer cell membrane and the secretion of matrix degrading proteases. Osteoblasts modulate the deposition of bone mineral components and secrete extracellular matrix proteins.

Objectives

To investigate the ability of osteoblasts and osteosarcoma to secrete acid and express matrix degrading proteases upon metabolic activation. To examine also the potential contribution of vATPases to proton secretion expressed on osteoblasts.

Methods

Osteoblasts were isolated from trabecular bone and characterised by reverse transcriptase-polymerase chain reaction and

SUBMITTER: George M 

PROVIDER: S-EPMC1754733 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature