Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study.

ABSTRACT:

Background

Cryopreservation of peripheral blood mononuclear cells has been used to preserve and standardize immunologic measurements for multicenter studies, however, effects of cryopreservation on cytokine responses are incompletely understood. In designing immunologic studies for a new multicenter birth cohort study of childhood asthma, we performed a series of experiments to determine the effects of two different methods of cryopreservation on the cytokine responses of cord and peripheral blood mononuclear cells.

Results

Paired samples of PBMC were processed freshly, or after cryopreservation in a Nalgene container (NC) or a controlled-rate freezer (CRF). Although there were some differences between the methods, cryopreservation inhibited PHA-induced IL-10 secretion and D

SUBMITTER: Shreffler WG 

PROVIDER: S-EPMC1762025 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature