'Fractional recovery' analysis of a presynaptic synaptotagmin 1-anchored endocytic protein complex.

ABSTRACT:

Background

The integral synaptic vesicle protein and putative calcium sensor, synaptotagmin 1 (STG), has also been implicated in synaptic vesicle (SV) recovery. However, proteins with which STG interacts during SV endocytosis remain poorly understood. We have isolated an STG-associated endocytic complex (SAE) from presynaptic nerve terminals and have used a novel fractional recovery (FR) assay based on electrostatic dissociation to identify SAE components and map the complex structure. The location of SAE in the presynaptic terminal was determined by high-resolution quantitative immunocytochemistry at the chick ciliary ganglion giant calyx-type synapse.

Methodology/principle findings

The first step in FR analysis was to immunoprecipitate (IP) the complex with an antibody aga

SUBMITTER: Khanna R 

PROVIDER: S-EPMC1762330 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature