Probe selection and expression index computation of Affymetrix Exon Arrays.

ABSTRACT:

Background

There is great current interest in developing microarray platforms for measuring mRNA abundance at both gene level and exon level. The Affymetrix Exon Array is a new high-density gene expression microarray platform, with over six million probes targeting all annotated and predicted exons in a genome. An important question for the analysis of exon array data is how to compute overall gene expression indexes. Because of the complexity of the design of exon array probes, this problem is different in nature from summarizing gene-level expression from traditional 3' expression arrays.

Methodology/principal findings

In this manuscript, we use exon array data from 11 human tissues to study methods for computing gene-level expression. We showed that for most genes there i

SUBMITTER: Xing Y 

PROVIDER: S-EPMC1762343 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature