Members of the HRT family of basic helix-loop-helix proteins act as transcriptional repressors downstream of Notch signaling.

ABSTRACT: The Hairy-related transcription-factor (HRT) genes encode three related basic helix-loop-helix transcription factors that show sequence similarity to the Hairy and Enhancer of split family of transcriptional repressors. HRT proteins are expressed in specific regions of the developing heart, vasculature, pharyngeal arches and somites, and the periodicity of their expression in somitic precursors mirrors that of Notch signaling-related molecules. In the present study, we show that the intracellular domain of the Notch1 receptor (Notch1 IC), which is constitutively active, up-regulates HRT2 expression in 10T(1/2) fibroblasts. Luciferase reporter assays using the regulatory regions of the mouse HRT genes revealed that transcription of all three genes is stimulated by Notch1 IC. The promoters o

SUBMITTER: Nakagawa O 

PROVIDER: S-EPMC17631 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature