The DNA-damage signature in Saccharomyces cerevisiae is associated with single-strand breaks in DNA.

ABSTRACT:

Background

Upon exposure to agents that damage DNA, Saccharomyces cerevisiae undergo widespread reprogramming of gene expression. Such a vast response may be due not only to damage to DNA but also damage to proteins, RNA, and lipids. Here the transcriptional response of S. cerevisiae specifically induced by DNA damage was discerned by exposing S. cerevisiae to a panel of three "radiomimetic" enediyne antibiotics (calicheamicin gamma1I, esperamicin A1 and neocarzinostatin) that bind specifically to DNA and generate varying proportions of single- and double-strand DNA breaks. The genome-wide responses were compared to those induced by the non-selective oxidant gamma-radiation.

Results

Given well-controlled exposures that resulted in similar and minimal cell death (~20-25%) acr

SUBMITTER: Fry RC 

PROVIDER: S-EPMC1764021 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature